Journal:

Article Title: Coordinate Intracellular Expression of Salmonella Genes Induced during Infection

doi:

Figure Lengend Snippet: Intracellular expression of ivi genes in cultured macrophages and cultured epithelial cells. Individual ivi fusions were incubated with cultured RAW 264.7 murine macrophages and cultured human epithelial cell lines HEp-2 (larynx carcinoma) and Henle-407 (embryonic small intestine). The coculture was incubated for 30 min, washed, treated with gentamicin to kill extracellular bacteria, washed, and incubated for 4 h. The cultured mammalian cells were lysed with Triton X-100, and β-galactosidase assays were performed on the recovered intracellular bacteria. For comparison, β-galactosidase assays were also performed on individual ivi fusions grown for 4 h in LB and in MEM supplemented with 10% FCS. Preselected Lac− and Lac+ strains were obtained from the initial nonselected pool of integrated IVET fusions. Error bars represent 1 standard deviation of the measured value.

Article Snippet: The murine macrophage cell line RAW 264.7 and human epithelial cell lines HEp-2 (larynx carcinoma) and Henle-407 (embryonic small intestine) (ATCC TIB-71, CCL-23, and CCL-6, respectively) were obtained from the American Type Culture Collection, Rockville, Md., and maintained in minimum essential medium (MEM) supplemented with Earle’s salts, l -glutamine, and 10% heat-inactivated fetal calf serum (FCS) (Life Technologies, Rockville, Md.).

Techniques: Expressing, Cell Culture, Incubation, Standard Deviation